Urinary incontinence is one of the complications of spinal cord injury that affects different aspects of a person's life and has many negative consequences. This study investigated the effect of clean intermittent self-catheterization on the anxiety and quality of life of patients. This research was a semi-experimental before-after study with a control group and 80 spinal cord injury patients were studied. The samples were selected as available and were assigned to two intervention groups and a control group using a regular method. Beck's anxiety questionnaire and 23-question quality of life questionnaire of spinal cord injury patients were used to collect data. Data were analyzed using SPSS version 23 and Medcalc version 8. The findings showed that before the intervention, the two groups were homogeneous in terms of quality of life and anxiety and the research intervention (clean intermittent survey) led to a significant increase in quality of life (P = 0.0001) and a significant decrease in anxiety (P = 0.0001). According to the results obtained from the present study, clean intermittent self-catheterization by the patient can be used as a method to increase the quality of life of patients and reduce their anxiety.